4M7G
Streptomyces Erythraeus Trypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.958, 61.249, 74.149 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.220 - 0.810 |
| R-factor | 0.112 |
| Rwork | 0.109 |
| R-free | 0.12000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sgt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.032 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.380 | 0.830 |
| High resolution limit [Å] | 0.790 | 0.790 |
| Number of reflections | 234158 | |
| Completeness [%] | 99.8 | 96.9 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 298 | 10 mM calcium chloride, 100 mM HEPES pH 7.0, 2.72 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
