4M7F
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with bound ManNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9745 |
Spacegroup name | P 4 |
Unit cell lengths | 119.540, 119.540, 44.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.461 - 2.100 |
R-factor | 0.1939 |
Rwork | 0.187 |
R-free | 0.21390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Native FIBCD1 structure |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.1.4) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.461 | 53.461 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.069 | 0.033 | 0.174 |
Total number of observations | 5204 | 23101 | |
Number of reflections | 36910 | ||
<I/σ(I)> | 12.6 | 18 | 4.2 |
Completeness [%] | 99.8 | 99.1 | 100 |
Redundancy | 4.2 | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.7M Ammonium sulphate, 7% Dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |