4M6W
Crystal structure of the C-terminal segment of FANCM in complex with FAAP24
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796, 0.9795, 0.9792, 0.9568 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.174, 98.111, 140.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.2567 |
| Rwork | 0.254 |
| R-free | 0.29900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.068 | 0.502 |
| Number of reflections | 12155 | |
| <I/σ(I)> | 20.5 | 2.8 |
| Completeness [%] | 97.6 | 97.5 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 1.0M (NH4)2SO4, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
