4M5T
Disulfide trapped human alphaB crystallin core domain in complex with C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.010, 46.770, 77.710 |
Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
Resolution | 68.799 - 2.000 |
R-factor | 0.1954 |
Rwork | 0.193 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.241 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.799 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.059 | 0.023 | 0.365 |
Number of reflections | 24867 | ||
<I/σ(I)> | 14.1 | 33.71 | 3.48 |
Completeness [%] | 97.5 | 98.4 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.085 M MES, pH 6.5, 0.17 M ammonium sulfate, 25.5% PEG5000 MME, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |