4M59
Crystal structure of the pentatricopeptide repeat protein PPR10 in complex with an 18-nt psaJ RNA element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9067 |
Spacegroup name | P 43 |
Unit cell lengths | 83.428, 83.428, 226.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.870 - 2.460 |
R-factor | 0.261 |
Rwork | 0.260 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m57 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.999 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 54087 | |
Completeness [%] | 96.3 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 10% PEG 3350, 8% Tacsimate, 0.1M MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |