4M53

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDPCP

Replaces:  3SJY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	I 2 3
Unit cell lengths	186.891, 186.891, 186.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 2.000
R-factor	0.1612
Rwork	0.160
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aho
RMSD bond length	0.006
RMSD bond angle	1.143
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.108	0.491
Number of reflections	72719
<I/σ(I)>	12.77	2.73
Completeness [%]	99.6	98.6
Redundancy	5.01	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	301	25mM Tris-HCl, 400mM NaCl, 10mM 2-mercaptoethanol, 2M sodium formate, 50 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K