4M4Z
SH3 and SH2 domains of human Src-like adaptor protein 2 (SLAP2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-11 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.460, 42.430, 46.100 |
Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
Resolution | 28.445 - 2.100 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lck |
RMSD bond length | 0.004 |
RMSD bond angle | 0.757 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (0.1.29) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.170 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.068 | 0.243 |
Number of reflections | 8539 | |
<I/σ(I)> | 10.5 | 4.8 |
Completeness [%] | 97.5 | 95.5 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M Lithium chloride, 20% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |