4M4D
Crystal structure of lipopolysaccharide binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00599 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.282, 59.906, 119.932 |
| Unit cell angles | 90.00, 102.63, 90.00 |
Refinement procedure
| Resolution | 19.891 - 2.909 |
| R-factor | 0.2322 |
| Rwork | 0.230 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ewf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.671 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.068 | 0.272 |
| Number of reflections | 21741 | |
| <I/σ(I)> | 17.3 | 3.29 |
| Completeness [%] | 91.4 | 76.9 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 22% PEG 8000, 100mM MOPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
