4M35
Crystal structure of gated-pore mutant H126/141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 90.230, 90.230, 420.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.17618 |
| Rwork | 0.175 |
| R-free | 0.20638 |
| Structure solution method | Direct refinement against model PDB |
| Starting model (for MR) | 2z90 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.855 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.053 | 0.139 |
| Number of reflections | 41264 | |
| <I/σ(I)> | 25.6 | 11.7 |
| Redundancy | 7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 6.5 | 298 | 50mM MgCl2, 0.1M sodium cacodylate, 20% PEG3350 , pH 6.5, Microbatch under oil, temperature 298K |
