4M33
Crystal structure of gated-pore mutant H141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-01-26 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | H 3 2 |
Unit cell lengths | 90.670, 90.670, 419.691 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.220 |
R-factor | 0.17741 |
Rwork | 0.175 |
R-free | 0.21336 |
Structure solution method | Direct refinement against model PDB |
Starting model (for MR) | 2z90 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.892 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.330 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.134 | 0.328 |
Number of reflections | 33223 | |
<I/σ(I)> | 15.6 | 6.9 |
Redundancy | 11.1 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 6.5 | 298 | 250mM MgCl2, 0.1M sodium cacodylate, 20% PEG3350, pH 6.5, Microbatch under oil, temperature 298K |