4M2L

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in nucleotide-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	P 31 2 1
Unit cell lengths	95.815, 95.815, 165.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.908 - 2.149
R-factor	0.1904
Rwork	0.188
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aho
RMSD bond length	0.007
RMSD bond angle	1.127
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.990	2.250
High resolution limit [Å]	2.149	2.150
Rmerge	0.057	0.374
Number of reflections	48518
<I/σ(I)>	18.01	3.83
Completeness [%]	99.9	99.7
Redundancy	5.86	5.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	301	25mM Tris-HCl, 400mM NaCl, 10mM 2-mercaptoethanol, 25mM Na-HEPES, 25mM MgSO4, 0.8M Li2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K