4M2A
Crystal structure of the udp-glucose pyrophosphorylase from Leishmania major in the post-reactive state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8055 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.790, 89.090, 136.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.660 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oeg |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.600 |
| High resolution limit [Å] | 1.660 |
| Rmerge | 0.068 |
| Number of reflections | 57313 |
| <I/σ(I)> | 28.78 |
| Completeness [%] | 99.7 |
| Redundancy | 14.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 0.1M BIS-TRIS, 22% W/V PEG-MME- 2000, 20MM (NH4)2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP |
