4M1P
Crystal structure of the copper-sensing repressor CsoR with Cu(I) from Geobacillus thermodenitrificans NG80-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2013-05-09 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 89.204, 89.204, 57.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.375 - 2.564 |
| R-factor | 0.2173 |
| Rwork | 0.214 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4adz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.067 | 0.800 |
| Number of reflections | 4463 | |
| <I/σ(I)> | 30.91 | 1.94 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.3 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 2.8M sodium formate, 0.1M HEPES, 0.1M betaine hydrochloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
