4M1H
X-ray crystal structure of Chlamydia trachomatis apo NrdB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2012-04-06 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.975, 97.394, 99.237 |
Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
Resolution | 29.748 - 1.695 |
R-factor | 0.1809 |
Rwork | 0.179 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1syy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.748 | |
High resolution limit [Å] | 1.695 | 1.695 |
Number of reflections | 155357 | |
<I/σ(I)> | 28.6 | |
Completeness [%] | 99.7 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M sodium acetate, 10% PEG3000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |