4M19
dihydrodipicolinate synthase from C. jejuni with pyruvate bound to the active site and Lysine bound to allosteric site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.640, 96.990, 79.560 |
| Unit cell angles | 90.00, 111.53, 90.00 |
Refinement procedure
| Resolution | 48.495 - 2.000 |
| R-factor | 0.1671 |
| Rwork | 0.165 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ly8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 | |
| High resolution limit [Å] | 1.710 | 5.120 | 1.710 |
| Rmerge | 0.082 | 0.012 | 0.012 |
| Number of reflections | 93713 | 4574 | 6498 |
| <I/σ(I)> | 13.82 | 49.86 | 0.86 |
| Completeness [%] | 78.7 | 99.2 | 33.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.5 | 288 | 35% PEG3350, 0.3 M NH4SO4, 0.1 mM TRIS, pH 8.5, hanging drop, temperature 288K |
