4M0V

Crystal structure of E.coli SbcD with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Collection date	2013-06-20
Wavelength(s)	0.97880
Spacegroup name	P 1
Unit cell lengths	62.162, 69.229, 95.187
Unit cell angles	72.24, 84.00, 83.48

Refinement procedure

Resolution	19.967 - 1.830
R-factor	0.1636
Rwork	0.162
R-free	0.19860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lty
RMSD bond length	0.007
RMSD bond angle	1.132
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.830	1.830
Number of reflections	128912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K