4M0I
CRYSTAL STRUCTURE OF SYNTHETIC HIV-1 CAPSID C-TERMINAL DOMAIN (CTD) C198S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97530 |
| Spacegroup name | I 41 |
| Unit cell lengths | 59.001, 59.001, 61.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.610 - 2.800 |
| R-factor | 0.23893 |
| Rwork | 0.236 |
| R-free | 0.29379 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lry |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.573 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.057 | 0.669 |
| Number of reflections | 2648 | |
| <I/σ(I)> | 30.7 | 2.2 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 2 M ammonium sulfate, 100 mM sodium cacodylate pH 6.5, and 200 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
