4M04

Human DNA Polymerase Mu ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2012-10-08
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.892, 68.467, 109.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.174 - 1.898
R-factor	0.1654
Rwork	0.164
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2IHM (molecule B)
RMSD bond length	0.010
RMSD bond angle	1.325
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.898	4.090	1.898
Rmerge		0.042	0.316
Number of reflections	36290
<I/σ(I)>	13.6
Completeness [%]	99.7	99.3	98.1
Redundancy	6.2	7.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Crystals were grown by mixing 1uL of protein/DNA mixture with 1uL mother liquor (85-90mM HEPES pH 7.5, 17-18% PEG4000), using the sitting drop vapor diffusion technique. Crystals were then transferred to a cryoprotectant solution containing 0.1M HEPES pH 7.5, 10mM MgCl2, 50mM NaCl, 20% PEG4000, 5% glycerol, 1mM dUMPNPP in two steps. , VAPOR DIFFUSION, SITTING DROP, temperature 295K