4M02
Middle fragment(residues 494-663) of the binding region of SraP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.984, 46.461, 47.594 |
| Unit cell angles | 90.00, 102.64, 90.00 |
Refinement procedure
| Resolution | 46.440 - 1.590 |
| R-factor | 0.16576 |
| Rwork | 0.164 |
| R-free | 0.20208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m01 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.590 | 1.600 |
| Rmerge | 0.103 | 0.323 |
| Number of reflections | 18581 | |
| <I/σ(I)> | 10.034 | 3.726 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M Tris, 2.0M Ammonium phosphate monobasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
