4M01
N terminal fragment(residues 245-575) of binding region of SraP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.704, 179.644, 71.510 |
| Unit cell angles | 90.00, 89.77, 90.00 |
Refinement procedure
| Resolution | 44.911 - 2.100 |
| R-factor | 0.1596 |
| Rwork | 0.158 |
| R-free | 0.19510 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.337 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.158 | 0.357 |
| Number of reflections | 99475 | |
| <I/σ(I)> | 14.346 | 5.63 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 10% PEG 6000, 0.1M MES, 1.0M lithium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
