4LZO

Crystal structure of human PRS1 A87T mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	H 3
Unit cell lengths	170.402, 170.402, 62.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.180 - 3.310
R-factor	0.26552
Rwork	0.263
R-free	0.31183
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.141
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.200
High resolution limit [Å]	3.310	3.310
Number of reflections	10020

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.1	295	pH 4.1, VAPOR DIFFUSION, temperature 295K