4LZD

Human DNA polymerase mu- Apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2012-09-03
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	124.192, 124.192, 50.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.856 - 1.849
Rwork	0.181
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2IHM (molecule B) was used as the starting molecule for molecular replacement for a lower resolution structure which was then used as the starting model for the current higher resolution structure.
RMSD bond length	0.011
RMSD bond angle	1.280
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.849	3.990	1.850
Rmerge		0.037	0.414
Number of reflections	34121
<I/σ(I)>	16.4
Completeness [%]	99.3	99.5	96.4
Redundancy	9	13.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	Crystals were grown by mixing 1 uL of concentrated protein (10.7mg/mL) with 1 uL of mother liquor (50 mM imidazole pH 8, 0.8M sodium citrate) at room temperature, using the hanging drop vapor diffusion technique. The crystals reached usable size within 24 hours, and were then directly transferred to a cryoprotectant solution containing 50mM imidazole pH 8, 75mM NaCl, 1M sodium citrate, 10% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 295K