4LYQ
Crystal Structure of Glycoside Hydrolase Family 5 Mannosidase from Rhizomucor miehei, E202A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.173, 76.549, 54.836 |
| Unit cell angles | 90.00, 113.36, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.12685 |
| Rwork | 0.125 |
| R-free | 0.16326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uuq |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.177 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.600 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | |
| Number of reflections | 31831 | |
| Completeness [%] | 94.5 | 63.1 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 16% PEG 4000, 0.1M HEPES buffer pH 7.5, 5% mannotriose, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
