4LY7
Ancestral RNase H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 61 |
| Unit cell lengths | 82.379, 82.379, 44.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.189 - 1.360 |
| R-factor | 0.1279 |
| Rwork | 0.126 |
| R-free | 0.15590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rn2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.189 | 1.410 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 37194 | |
| <I/σ(I)> | 26.1 | 2.7 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 20% PEG 3350, 30mM lithium sulfate, 1mM TCEP, 100mM Bis-Tris pH 6.5, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
