4LY6

Nucleotide-induced asymmetry within ATPase activator ring drives s54-RNAP interaction and ATP hydrolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	119.339, 130.037, 206.368
Unit cell angles	90.00, 89.73, 89.90

Refinement procedure

Resolution	37.855 - 3.600
R-factor	0.2603
Rwork	0.258
R-free	0.30770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.087
Data reduction software	d*TREK (9.7 W8RSSI)
Data scaling software	d*TREK (9.7 W8RSSI)
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.860	37.860	3.730
High resolution limit [Å]	3.600	7.740	3.600
Rmerge	0.084	0.038	0.363
Number of reflections	109790
<I/σ(I)>	5	13.4	1.2
Completeness [%]	76.4	84.8	44.3
Redundancy	1.66	1.66	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	277	20% ethylene glycol, pH 7.9, vapor diffusion, hanging drop, temperature 277K