4LY5
Crystal structure of NaASP2 with Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-12 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.100, 51.860, 43.580 |
| Unit cell angles | 90.00, 113.88, 90.00 |
Refinement procedure
| Resolution | 31.600 - 1.664 |
| R-factor | 0.19298 |
| Rwork | 0.191 |
| R-free | 0.23269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u53 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.013 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.058 | 1.832 |
| Number of reflections | 18302 | |
| <I/σ(I)> | 13.5 | 0.8 |
| Completeness [%] | 99.8 | 96.5 |
| Redundancy | 6.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 100mM imidazole, 40% PEG 400, 10mM ZnCl2 , pH 8.5, VAPOR DIFFUSION, SITTING DROP |
