4LX9
Archaeal amino-terminal acetyltransferase (NAT) bound to acetyl coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-01 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.840, 35.062, 51.219 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 21.049 - 1.980 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.178 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.133 | 0.460 |
| Number of reflections | 12433 | |
| <I/σ(I)> | 7.66 | 1.9 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 3350, 0.1 M zinc acetate, Protein: 7.5 mg/mL, 20 mM tris, 150 mM NaCl, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
