4LX1
Crystal structure of Myo5a globular tail domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.080, 94.580, 135.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.000 - 1.870 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lx0 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.245 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.980 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.360 | |
Number of reflections | 83459 | |
<I/σ(I)> | 4.89 | |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MES pH6 12% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |