4LX0
Crystal structure of Myo5b globular tail domain in complex with active Rab11a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.100, 125.920, 157.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.500 - 2.190 |
R-factor | 0.1829 |
Rwork | 0.181 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.328 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.500 | 2.320 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 98156 | |
Completeness [%] | 99.8 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10 % peg, 90 mM NaF, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |