4LWT
The 1.6A Crystal Structure of Humanized Xenopus MDM2 with RO5027344
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.066, 67.976, 67.168 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.380 - 1.600 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.380 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.047 | 0.361 |
Number of reflections | 13260 | |
<I/σ(I)> | 20.3 | 4.6 |
Completeness [%] | 98.9 | 98.1 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 40-50% Saturated Ammonium Sulfate, 0.1M MES, pH 6.5, 5% PEG200, VAPOR DIFFUSION, HANGING DROP, temperature 278K |