4LWP

Crystal structure of PRMT6-SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	101.428, 108.465, 137.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.596 - 2.353
R-factor	0.2068
Rwork	0.204
R-free	0.24930
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.198
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	31792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	100 mM Citrate pH 5.5, 20% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 293K