4LW9
Crystal structure of Vibrio cholera major pseudopilin EpsG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0712 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.610, 70.015, 131.544 |
Unit cell angles | 90.00, 103.29, 90.00 |
Refinement procedure
Resolution | 29.540 - 1.900 |
R-factor | 0.20468 |
Rwork | 0.202 |
R-free | 0.24527 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.323 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.540 |
High resolution limit [Å] | 1.900 |
Number of reflections | 117230 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 6 mM zinc sulfate, 60 mM MES pH 6.5, 15% PEG MME 550, VAPOR DIFFUSION, HANGING DROP, temperature 278K |