4LW6
Crystal structure of catalytic domain of Drosophila beta1,4galactosyltransferase 7 complex with xylobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.943, 81.943, 133.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.004 - 2.400 |
R-factor | 0.1789 |
Rwork | 0.177 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lw6 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.557 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 17496 | |
<I/σ(I)> | 21.2 | 2 |
Completeness [%] | 94.8 | 77 |
Redundancy | 7.6 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 280 | 100 mM HEPES, 5% PEG 6000, 10% MPD, 100 mM Serinol, 10 mM xylobiose, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 280K |