Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.982, 78.506, 55.138 |
| Unit cell angles | 90.00, 113.24, 90.00 |
Refinement procedure
| Resolution | 39.674 - 2.002 |
| R-factor | 0.2191 |
| Rwork | 0.216 |
| R-free | 0.28060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c2m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.178 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.058 | 0.037 | 0.405 |
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 93.0 | 97.9 | 97.8 |
| Redundancy | 3.5 | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50mM Imidazole, 350mM NaCl, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
