4LVJ
MobM Relaxase Domain (MOBV; Mob_Pre) bound to plasmid pMV158 oriT DNA (22nt). Mn-bound crystal structure at pH 5.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87260 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.020, 112.020, 90.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.220 - 2.170 |
R-factor | 0.20322 |
Rwork | 0.201 |
R-free | 0.24116 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.784 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.290 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.150 | |
Number of reflections | 16928 | |
<I/σ(I)> | 7.3 | |
Completeness [%] | 98.2 | 100 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 21% PEG 6000, 0.1M Sodium Acetate pH4.6. Cryo-protecting/soaking at pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |