4LV5
Murine IRGa6 bound to Toxoplasma ROP5B, a pseudokinase GDI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 |
Unit cell lengths | 52.600, 54.700, 85.600 |
Unit cell angles | 99.00, 106.60, 106.90 |
Refinement procedure
Resolution | 39.328 - 1.700 |
R-factor | 0.1941 |
Rwork | 0.192 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3Q60 and then 1TPZ:A using phaser |
RMSD bond length | 0.008 |
RMSD bond angle | 1.106 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.330 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.058 | 0.018 | 0.018 |
Number of reflections | 92554 | 1028 | 6860 |
<I/σ(I)> | 19.19 | 113.1 | 1.15 |
Completeness [%] | 99.5 | 98.1 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% PEG 3350, 0.1M Ammonium Citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |