4LUN
Structure of the N-terminal mIF4G domain from S. cerevisiae Upf2, a protein involved in the degradation of mRNAs containing premature stop codons
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.813, 50.927, 71.993 |
Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
Resolution | 41.183 - 1.641 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.22970 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.006 |
RMSD bond angle | 0.993 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Arcimboldo |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 36059 | |
<I/σ(I)> | 14.6 | 2.3 |
Completeness [%] | 98.4 | 95.9 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100mM Tris-HCl pH 8.5 and 30% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K |