4LUK
The crystal structure of the P132A, Y133G mutant of Pyrococcus furiosus phosphoglucose isomerase in complex with manganese and 5-phospho-D-arabinonate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.850, 43.280, 58.450 |
| Unit cell angles | 90.00, 122.90, 90.00 |
Refinement procedure
| Resolution | 28.920 - 1.410 |
| R-factor | 0.15336 |
| Rwork | 0.150 |
| R-free | 0.21388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x7n |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.064 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.920 | 1.440 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.036 | 0.349 |
| Number of reflections | 32797 | |
| <I/σ(I)> | 13 | 2.1 |
| Completeness [%] | 91.2 | 55.4 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 290 | 0.35 M MgCl2, 0.1 M sodium acetate PH 5.5 and 19% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
