4LTR
Bacterial sodium channel, His245Gly mutant, I222 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.70192 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 157.205, 161.384, 165.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 5.800 |
R-factor | 0.26519 |
Rwork | 0.262 |
R-free | 0.31762 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lto |
RMSD bond length | 0.007 |
RMSD bond angle | 0.966 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 6.110 |
High resolution limit [Å] | 5.800 | 5.800 |
Rmerge | 0.130 | 4.181 |
Number of reflections | 5978 | |
<I/σ(I)> | 0.7 | 0.7 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 13.1 | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 28% PEG400, 100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |