4LTB
Coiled-coil domain of TRIM25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.669, 83.158, 92.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.209 - 2.590 |
| R-factor | 0.2082 |
| Rwork | 0.203 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((AutoMR)) |
| Refinement software | PHENIX ((phenix.refine: dev_1427)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 13941 | |
| <I/σ(I)> | 17.3 | 2.2 |
| Completeness [%] | 96.9 | 81.4 |
| Redundancy | 13.1 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 6-12% PEG 4000, 10 mM sodium acetate, pH 5.3-6.6, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
