4LT7

Crystal structure of the c2a domain of rabphilin-3a in complex with a calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2012-05-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5417
Spacegroup name	P 21 21 21
Unit cell lengths	38.690, 40.030, 90.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.24531
Rwork	0.243
R-free	0.28943
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2chd
RMSD bond length	0.005
RMSD bond angle	0.934
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.143	0.515
Number of reflections	3660
<I/σ(I)>	3.6	1.9
Completeness [%]	72.3	68.3
Redundancy	2.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	10 mM CaCl2, 150mM NaCl, 25mM HEPES ph 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K