4LSV
Crystal structure of broadly and potently neutralizing antibody 3BNC117 in complex with HIV-1 clade C C1086 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 191.615, 191.615, 103.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.320 - 3.000 |
| R-factor | 0.262 |
| Rwork | 0.260 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.589 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.020 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.091 | 0.524 |
| Number of reflections | 22670 | |
| <I/σ(I)> | 17.46 | 3.32 |
| Completeness [%] | 98.5 | 95.5 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 0.1M imidazole, 11% PEG 3350, 0.5M NaCl, 0.2M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
