4LSK
Crystal Structure of tRNA Proline (CGG) Bound to Codon CCG-G on the Ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-23 |
| Detector | ADSC q315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.252, 448.398, 624.576 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.972 - 3.480 |
| R-factor | 0.231833199846 |
| Rwork | 0.230 |
| R-free | 0.26991 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.083 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.133 | 3.400 | |
| High resolution limit [Å] | 3.310 | 14.800 | 3.310 |
| Rmerge | 0.321 | 0.014 | 0.014 |
| Number of reflections | 854421 | 9125 | 61120 |
| <I/σ(I)> | 4.59 | 18.16 | 1.04 |
| Completeness [%] | 99.4 | 88.7 | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K |
