4LSF

Ion selectivity of OmpF soaked in 0.1M KBr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91956
Spacegroup name	P 3
Unit cell lengths	116.632, 116.632, 51.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.158 - 1.900
R-factor	0.2687
Rwork	0.267
R-free	0.29850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2omf
RMSD bond length	0.015
RMSD bond angle	1.503
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.098	0.787
Number of reflections	61382
<I/σ(I)>	20	1.8
Completeness [%]	100.0	100
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50% PEG200, 0.1 M sodium cacodylate, 0.2 M magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K