4LSD
Myokine structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.200, 93.200, 284.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.520 - 2.280 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.23600 |
| Structure solution method | SIRAS |
| Starting model (for MR) | thimerosal derivative |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.520 |
| High resolution limit [Å] | 2.280 |
| Number of reflections | 53502 |
| Completeness [%] | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | citrate, cacodylate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
