4LS5
Crystal structure of beta-ketoacyl-ACP synthase II (FabF) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.513, 87.522, 144.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.977 - 1.803 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gqd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.879 | 28.977 | 1.900 |
| High resolution limit [Å] | 1.803 | 5.700 | 1.800 |
| Rmerge | 0.053 | 0.024 | 0.551 |
| Total number of observations | 9695 | 40924 | |
| Number of reflections | 85353 | ||
| <I/σ(I)> | 13.4 | 23.8 | 1.4 |
| Completeness [%] | 99.8 | 98.3 | 99.1 |
| Redundancy | 3.5 | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 10% PEG3350, 0.25 M KCl , pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
