4LQY

Crystal Structure of Human ENPP4 with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07500
Spacegroup name	C 1 2 1
Unit cell lengths	181.533, 51.143, 52.741
Unit cell angles	90.00, 102.38, 90.00

Refinement procedure

Resolution	37.852 - 1.540
R-factor	0.1424
Rwork	0.140
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsu
RMSD bond length	0.009
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.540
High resolution limit [Å]	1.500	3.610	1.500
Rmerge	0.064	0.040	0.662
Number of reflections	74645
<I/σ(I)>	14.7
Completeness [%]	98.9	97.3	94.2
Redundancy	3.9	4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	293	200 mM ammonium citrate dibasic, 17.5% to 19.5% (w/v) PEG 3350, pH 6.5, hanging drop, temperature 293K