4LP4

Crystal structure of the human RAGE VC1 fragment in space group P62

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-18
Detector	MAR CCD 165 mm
Wavelength(s)	1.04
Spacegroup name	P 62
Unit cell lengths	102.000, 102.000, 103.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.387 - 2.400
R-factor	0.1805
Rwork	0.178
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o3u
RMSD bond length	0.006
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000		2.500
High resolution limit [Å]	2.400	20.000	2.400
Rmerge	0.075	0.025	0.494
Number of reflections	23876	33	2763
<I/σ(I)>	22.72	40.57	4.77
Completeness [%]	99.7	71.7	100
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	2.5 M sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K