4LP1
Crystal structure of CyaY protein from Psychromonas ingrahamii in complex with Eu(III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.821, 49.992, 46.580 |
| Unit cell angles | 90.00, 90.43, 90.00 |
Refinement procedure
| Resolution | 31.147 - 1.803 |
| R-factor | 0.2158 |
| Rwork | 0.213 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hs5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.235 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.039 | 0.019 | 0.298 |
| Number of reflections | 30293 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 91.7 | 83.3 | 89.8 |
| Redundancy | 1.6 | 1.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 297 | 20mM sodium acetate, 200mM MgCl2, 29.5% PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
