4LOW
Structure and identification of a pterin dehydratase-like protein as a RuBisCO assembly factor in the alpha-carboxysome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 |
Unit cell lengths | 51.110, 51.110, 60.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.110 - 1.300 |
R-factor | 0.2025 |
Rwork | 0.203 |
R-free | 0.20360 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 51.110 |
High resolution limit [Å] | 1.300 |
Number of reflections | 36178 |
Completeness [%] | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 17.5% PEG3350, 800 mM ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |